Demultiplexing Reads

Overview

Teaching: 0 min
Exercises: 0 min

Questions

• How do I demultiplex reads from different samples?

Objectives

• Understand how read barcodes function

• Demultiplex reads from several samples into separate files

Exercise: Demultiplex a small set of reads by hand Exercise: Use a computational tool (FIXME: Which?) to demultiplex a small dataset. (Does not have to be the same one as later lesson steps.)

Key Points

• Barcodes let us sequence many samples at the same time and separate them computationally

• Most pipelines require demultiplexing reads as a first step